Laser capture microscopy
نویسندگان
چکیده
منابع مشابه
Extended Field Laser Confocal Microscopy (EFLCM): Combining automated Gigapixel image capture with in silico virtual microscopy
BACKGROUND Confocal laser scanning microscopy has revolutionized cell biology. However, the technique has major limitations in speed and sensitivity due to the fact that a single laser beam scans the sample, allowing only a few microseconds signal collection for each pixel. This limitation has been overcome by the introduction of parallel beam illumination techniques in combination with cold CC...
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Behind the exponential expansion of the acquired immunodeficiency syndrome (AIDS) epidemic, there is a continuous and progressive molecular evolution of human immunodeficiency virus (HIV)-1. In this regard, the molecular analysis of viral strains infecting several anatomic compartments in humans has become critical to understanding AIDS-related pathologies and to improving emerging therapeutic ...
متن کاملLaser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling
Laser capture microscopy (LCM) coupled with global transcriptome profiling could enable precise analyses of cell populations without the need for tissue dissociation, but has so far required relatively large numbers of cells. Here we report a robust and highly efficient strategy for LCM coupled with full-length mRNA-sequencing (LCM-seq) developed for single-cell transcriptomics. Fixed cells are...
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The sequencing of the human genome began with much fanfare in 1990 with the start of the Human Genome Project. Major portions of that program have been completed since the interim draft of the human sequence was published in 2001 [1]. Scientists now turn their attention to an even more complex and arduous task, characterizing the human ‘proteome’. The term proteome was coined to describe all th...
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ژورنال
عنوان ژورنال: Molecular Pathology
سال: 2000
ISSN: 1366-8714
DOI: 10.1136/mp.53.2.64